Subjects of the study were camels and humans in close contact with camels; those included: camel rearing communities, Veterinarians and slaughter houses labours. This study was designed to investigate the possibility of infection of camel rearing communities with T. Four Sudanese States were chosen as study areas for this study on the light of the density of camel population. A total of 1186 blood samples were collected from camels and 336 blood samples from humans. Combination of parasitological, serological and advanced molecular diagnosis... evansi infection using parasitological, serological and molecular tests. evansi in the study areas in the Sudan using classical and new diagnostic techniques of molecular biology include: Polymerase Chain Reaction (PCR) and Loop mediated-isothermal Amplification (LAMP). None of the human samples were positive parasitologically using Giemsa stained slides. All samples were screened for T. Whereas 23 camel samples were positive (1.94%).